Automation in Structural Biology Beamlines of the Photon Factory
نویسندگان
چکیده
منابع مشابه
The Evolution of the ESRF's Structural Biology Beamlines
Structural Biologists are tackling ever more ambitious projects, for example more complex membrane proteins and larger macromolecular assemblies (Figure 1). Such systems often show considerable inter and intra crystal variation in diffraction quality. Sample evaluation prior to data collection, already widespread in Macromolecular Crystallography (Figure 2), will thus become more crucial as wil...
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Sample-exchange robots that can exchange cryo-pins bearing protein crystals out of experimental hutches according to user instructions have been developed. The robots were designed based on the SAM (Stanford Synchrotron Research Laboratory automated mounting) system. In order to reduce the time required for the sample exchange, the single tongs of the SAM system were modified and a double-tongs...
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Owing to recent advances in high-throughput technology in macromolecular crystallography beamlines, such as high-brilliant X-ray sources, high-speed readout detectors and robotics, the number of samples that can be examined in a single visit to the beamline has increased dramatically. In order to make these experiments more efficient, two functions, remote monitoring and diffraction image evalu...
متن کاملThe Upgrade Programme for the Structural Biology beamlines at the European Synchrotron Radiation Facility – High throughput sample evaluation and automation
Automation and advances in technology are the key elements in addressing the steadily increasing complexity of Macromolecular Crystallography (MX) experiments. Much of this complexity is due to the interand intra-crystal heterogeneity in diffraction quality often observed for crystals of multi-component macromolecular assemblies or membrane proteins. Such heterogeneity makes high-throughput sam...
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ژورنال
عنوان ژورنال: Nihon Kessho Gakkaishi
سال: 2007
ISSN: 0369-4585,1884-5576
DOI: 10.5940/jcrsj.49.359